Delivering genes efficiently and selectively into cells with low toxicity is necessary for the successful treatment of cancer, genetic diseases or viral infections. Viral vehicles are efficient in transfecting cells but suffer from many disadvantages like immunogenicity, difficult preparation or limited DNA loading capacity. Nonviral vectors like cationic liposomes deliver genes with lower efficiency, but they may be prepared in an uncomplicated and reproducible way, form stable complexes even with large DNA-molecules and they are not immunogenic. Novel cationic lipids were synthesized by double alkylation reaction of malonic esters with long chained alkyl halogenides or shorter 1,ω-dihalogenides. Saponification under alkaline conditions and decarboxylation of the alkylated malon esters lead to α-branched fatty acids or gemini fatty acids. After introducing variable oligo amine spacers, protected amino acids were added and afterwards deprotected, obtaining di- or tripeptide structures.