Anthropogenic organic chemicals, which partly persist in the wastewater treatment process and accumulate in the sewage sludge, enter the agro-environment by the use of sewage sludge as fertilizer. The possible uptake of these contaminants and their transfer into the food web may lead to a chronic exposure of humans and livestock. The metabolization of the incorporated compounds in plants may lead to the formation of unknown metabolites. In the present work the uptake and distribution of the typical sewage sludge contaminants triclosan (TCS), galaxolide®, and tonalide® by carrot, barley, and meadow fescue plants from spiked soils was investigated in greenhouse experiments and quantified by GC-MS. The metabolization of TCS was determined in a carrot cell culture and 8 phase II metabolites were elucidated by HPLC-MS. Subsequently, these metabolites could be determined in the roots of TCS exposed carrot plants. In additional studies, the metabolization of TCS in a horseradish hairy root culture was determined and 33 metabolites were detected by UPLC-QToF-MS. Structure proposals were elaborated for 23 metabolites, which were phase II and phase I+II metabolites.