Drought is one of the most severe stresses, limiting crop yields worldwide. A main objective of plant breeding, therefore, is the development of varieties withhigh yields under drought. Exotic plant material and efficient phenotyping protocols are needed to achieve this goal. Wild barley introgression lines (ILs) may facilitate the introduction of exotic alleles into elite breeding gene pools. In this thesis a set of wild barley introgression lines of the S42IL library was tested for its response to drought stress at the juvenile development stage. Quantitative trait loci (QTL) were localized for growth and physiological parameters. Two different studies were conducted. The first study was carried out at the high-throughput phenotyping platform ‘Plant Accelerator’ in Adelaide, Australia. The second study evaluated 52 S42ILs under severe drought stress under regular greenhouse conditions at Martin-Luther University in Halle, Germany. Additionally the S42ILs were genetically characterized with SNP markers generated by genotyping by sequencing (GBS).