The development of beta cells can be reproduced in vitro manipulating different signalling pathways. For example, embryonic stem cells can be differentiated into definitive endoderm and pancreatic progenitor cells by the addition of certain growth factors and small molecules. Thereby glucose is an important factor. In the following investigation, murine embryonic stem cell lines (CGR8, Sox17-DsRed) were differentiated into pancreatic progenitor cells analysing the influence of glucose. For successful differentiation, a concentration of at least 17.5 mM was indispensable. By the addition of very high concentrations of glucose (50 mM), an increased number of Pdx1 positive cells could be generated. The sensitive interval for adding high glucose was from day 3 to day 8 of differentiation. Other concentrations did not seem to have comparable effectiveness.