Type 2 Diabetes Mellitus is associated with DNA variations. The present study focused on the establishment of a strategy for the investigation of genetic predispositions and related mechanisms. Therefore, a work flow of 3 steps was established. The first step required the isolation of primary keratinocytes from plucked hair to obtain patient‐specific donor material. At second, the generation of induced pluripotent stem (iPS) cells wars necessary. Therefore, several non‐viral and nonintegrative reprogramming techniques were described. The third step aimed at the generation of pancreatic endocrine cells. To ensure an optimal pancreatic differentiation procedure, 3D co‐culture models with primary endothelial cells were applied. Detailed analysis was enabled by the use of transgenic murine embryonic stem cells and human embryonic stem cells, and human iPS cells. Together, the present study describes a strategy for the analysis of patient‐ and disease specific DNA variations.