Endocrine disruptors like the plasticizers DEHP and PCB (101+118) interfere with the hormonal balance. In the present study, reproductive and metabolic effects of DEHP and PCB were investigated in female C3H/N mice. Environmentally relevant doses of DEHP increased body weight and visceral fat deposits of female mice and their in utero and during lactation exposed F1 offspring. The exposure with DEHP and PCB together showed almost similar adipogenic effects. DEHP alone and in combination with PCB altered the expression profile of developmental markers in F2 preimplantation embryos, which have been exposed only via germ cells. PCB treatment showed no comparable adipogenic effects, but the gene expression profile of developmental markers were altered in F1 embryos. Together, dietary DEHP or DEHP and PCB at a critical ontogenetic window stimulated adipogenesis in female C3H/N mice and their offspring. Especially, DEHP showed strong negative effects on fertility and early embryo development.