Wheat production represents 25,4 % of the total cereal market. Increasing its production is a priority for food security programs worldwide. Winter wheat lines ectopically expressing a barley sucrose transporter (HOSUT) have increased sucrose uptake capacity and partitioning. Comprehensive analysis of HOSUT lines revealed an enhanced biomass in roots and shoots of seedlings and better performance of HOSUT lines in grain yield and thousand grain weight under field-like conditions. Omics analyses of the root system of HOSUT seedlings revealed an altered shikimate pathway and content of IAA and its precursors. Results in this study suggest a possible sucrose-triggered tryptophan-dependent auxin biosynthesis in young wheat roots. Furthermore, increased transcript levels of high and low affinity nitrate transporters in HOSUT seedling roots during N-depleted conditions suggest a sucrose/glucose mediated increased nitrogen use efficiency in HOSUT lines.