High glucose levels as they occur in diabetes mellitus favor the glycation of proteins and lead to the formation of AGEs. These AGEs accelerate aging processes and are associated with micro- and macrovascular complications as well as neurodegenerative diseases. Many effects are receptor-dependent, whereby RAGE is one of the most important interaction partners for AGEs. Treatment of Kelly neuroblastoma cells with MGO, the most potent precursor of AGE formation, did not lead to any reduction in cell viability. After treatment, there was an increased formation of CML and Western blot. Furthermore, glycated cells showed clear signals for CML and RAGE in flow cytometry. Glycation of matrix proteins resulted in the reduction of adhesion to laminin in the RTCA, with no effect on fibronectin and collagen IV. In addition, an increased migration rate could be detected by glycation of the cells.