The aim of this study was to investigate and compare the influence of different root canal dentin localizations (coronal, middle , apical) and endodontic irrigants (CHX, NaOCl, EDTA) on the microtensile bond strength to the root dentin in two systems, a self-etching resin cement (Bifix SE, Voco, Germany) and an established resin cement system (Bifix QM, Futurabond DC, Voco, Germany). A part of the coronal test bodies received a pretreatment with one of the rinse solutions.The microtensile bond strengths were measured using a universal testing machine. The highest bond strengths without the influence of a rinse solution could be achieved for Bifix SE at the coronal root dentin. There was no significant relationship between the determined bond strengths values and the different root canal sections (p ≥ 0,05; Tukey's Test).The endodontic irrigants CHX, NaOCl and their alternating rinse significantly reduced the adhesion values of Bifix SE. EDTA significantly increased the adhesion of Bifix QM (p≤0,05; Tukey's Test). Within the limitations of an in vitro investigation it can be concluded that the resin cement system as well as the applied irrigation solutions have a significant influence on the bond strength to the root canal dentin.