Nutrient deficiencies, but also a rising carbon dioxide concentration in the atmosphere are global stress factors that limit yield performance on today's crops. The most important task of plant breeding is the provision of suitable breeding material for plant production adapted to these stress conditions. In order to realize this goal, wild relatives of a corresponding crop plant, for example barley, are used. On their basis, wild barley introgression lines (ILs) are developed by introducing wild / exotic donor alleles into the elite barley gene pool. In the hydroculture experiments in the greenhouse the ILs and the control variety ‘Scarlett’ were analysed both under phosphorus or potassium deficiency compared to a standard nutrient solution as control. Both investigations revealed useful Hsp alleles for barley breeding. In growth chamber experiments the ILs were tested under 760 ppm compared to 380 ppm. In total, 31 QTLs (quantitative trait loci) confirmed Hsp effects already identified in previous hydroculture, greenhouse and field experiments with the same set of introgression lines.