This work examines whether different ECG recording methods affect the association of cardiovascular risk factors (CVRF) with HRV parameters. In 148 subjects of the CARLA study, 5 HRV parameters were determined from 4 different ECG recording methods using a controlled 20-minute ECG and a spontaneous 24-hour ECG. In order to detect effects of the ECG recording methods, normalized β-coefficients from linear regression analyses were compared with each other for 10 CVRF. In advance, a relevance limit was set to classify the results. The effects of ECG recording methods are clearly relevant for sex, nicotine consumption and hypertension with HRV parameters and occasionally relevant for age, diabetes mellitus type 2 and beta-blocker therapy. In the context of BMI, WHR and total / HDL cholesterol with HRV parameters, there are sporadically relevant influences of ECG recording methods, none during physical activity. The results show a CVRF-dependent trend for the influence of the ECG recording methods on the association of CVRF with HRV parameters. However, no significance was found.