Phosphatidylinositol-4,5-bisphosphat (PtdIns(4,5)P2) is a minor regulatory phospholipid in membranes of eukaryotes. Besides cytoplasmic or plasma membrane associated functions, human PtdIns(4,5)P2 also possesses nuclear functions like cell cycle regulation. In plants, overexpression of PI4P 5 kinases, enzymes synthezing PtdIns(4,5)P2, lead to an accumulation of PI4P 5-kinase in the nucleus. This study investigated the nuclear localization of Arabidopsis PIP5K2. PIP5K2 comprises two functional NLSs, NLSIV and NLSVI, which mediate nuclear transport of a cytosolic protein. Substitution of NLS IV in PIP5K2 (PIP5K2AAA) results in reduced nuclear localization. Additionally, PIP5K2 interacts with α-importins. In summary, the results suggest an active transport of PIP5K2 into the nucleus. Furthermore, remaining phenotypes in complementation studies of pip5k1 pip5k2 double mutant with PIP5K2AAA as well as interaction of PIP5K2 with cell cycle regulator Retinoblastoma related1 give hints towards nuclear functions of plant phosphoinositides.