The aim of this in vitro study was to evaluate the qualitative and quantitative treatment of various adhesive systems (Syntac®, Panavia F2.0, RealSeal™, Futurabond NR) to human root dentin. Under standardized conditions the specimens were prepared from 90 caries-free maxillary incisors and canines. According to their localization they were devided into coronal, middle and apical root canal third handled with adhesives and composite. The microtensile bond strengths were measured using a universal testing machine. The qualitative analysis was performed by scanning electron microscopy. At all root sections microtensile bond strengths were measured and decreased from coronal to apical. Within the groups testing Syntac® was a significant difference between coronal and middle and apical third (p ≤ 0,05; Tukey`s Test).The experiments with Panavia F2.0 revealed a significant difference between the coronal and apical root part (p ≤ 0,05, Tukey`s Test). Within the groups of RealSeal™ and Futurbond NR occurred no significant difference (p ≥ 0,05; Tukey`s Test). It can be concluded that the self- conditioning adhesives (Futurabond NR, RealSeal™), with low technique sensitivity, revealed high microtensile bond strengths otherwise the total etch products were influenced in their microtensile bond strength by missing intrinsic moisture.