The Al-Qilt catchment is located in the West Bank at the western side of the Jordan Valley covering about 173 km². About 97,000 inhabitants from Palestinian communities and Israeli colonies discharging about 14,000 m3/d of wastewater to the Al-Qilt catchment from which only about 30% is treated. This study aims to understand the occurrences, fate, transport and mobility of anthropogenic pollutants in order to identify the contaminated areas by using trace metals in sediment, wastewater and soil from Al-Qilt catchment. Several methods of investigation for sediment, soil and wastewater were applied. The BCR sequential fractionation gave indication about mobility or transport mainly for B, Cd, Sb, Pb, Cu, Hg and Sn. The sediments and topsoils of the Al-Qilt catchment are enriched clearly by anthropogenic pollutants due to discharge of raw wastewater, dumping sites, roadside and urban runoff, and sometimes due to natural effects. Therefore, trace metal inputs at Al-Qilt need to be kept under strict control in the future, since Ras Al-Qilt is considered as one of the most important springs in the area.